Kerala Forest
Research Institute invited sealed quotation from reputed vendors/ firms for
Metagenome sequencing of sandalwood- 18 samples.
Specifications :
·
Total Number
of samples-18
·
High quality
DNA Extraction and QC
·
Bacterial 16srRNA Library Preparation (V3-V4)
·
Amplicon metagenome sequencing using Illumina Miseq
·
Data generation-0.1 million high quality
reads per sample
·
Raw data in FASTQ format
·
Basic metagenome data analysis-Data QC,
quality-based filtering, OTU assignment, abundance-alpha diversity, beta
diversity
·
Data visualization (rarefaction curve, stacked
bar plots, heatmaps, PCOA
plots)
·
Report with detailed methodology, results and reference.
·
Total time for data generation: maximum 6 weeks
after receipt of the sample
·
Sample pick up should be arranged by the firm
·
Service provide should provide the raw data
along with the details of on the primer sequences used for the library
preparation, and details of sample index/barcodes sequences used for each
sample
·
The service provider should provide the DNA
QC, Library QC, Raw data, bioinformatics analysis reports.
Terms and conditions
1.
Quotations should
be sealed, super scribed as the ‘Quotation for the “Metagenome sequencing of
sandalwood” with quotation No. and date and should be submitted
to the Registrar, Kerala Forest Research Institute, Peechi, Thrissur-680
653, on or before 22-07-2024 latest by 3.00 PM
2.
Quotations will be
opened at 3.30 PM on the same day.
3.
Any Quotations
received after the date and time fixed is liable to be rejected.
4.
The rate quoted
should be valid for 90 days
5.
No representation
for enhancement of price once accepted will be considered during the period of
validity of the offer.
6.
The rate of GST
and additional charges if any should be shown separately.
7.
We have
DSIR Certificate valid up to 31-08-2024
8.
After completion
of the experiment all data files, output files and detailed report should be
handed over to the office of KFRI, Peechi.
9.
The maximum period
required for delivery of the item should be mentioned in the quotation.
10.
KFRI reserves the
right to reject any or all of the Tenders without assigning any reasons
thereof. In case of any dispute the jurisdiction shall be Ernakulam District,
Kerala.
S/d
REGISTRAR i/c
Date of notification: July 8, 2024 | July 22, 2024 |